Genomic-Assisted Marker Development Suitable for CsCvy-1 Selection in Cucumber Breeding
نویسندگان
چکیده
Cucumber is a widely grown vegetable crop plant and host to many different pathogens. vein yellowing virus (CVYV) causes economic losses on cucumber crops in Mediterranean countries some part of India such as West Bengal African Sudan. CVYV an RNA potyvirus transmitted mechanically by whitefly ( Bemisia tabaci ) semipersistent manner. Control this heavily dependent the management insect vector breeding virus-resistant lines. DNA markers have been used conventional programs via marker-assisted selection (MAS). However, very few resistance sources against exist, also lack tightly linked molecular these restricts rapid generation resistant In work, we genomics coupled with bulked segregant analysis method generated MAS-friendly Kompetitive allele specific PCR (KASP) suitable for CsCvy-1 using segregating F 2 mapping population commercial Variant was performed generate single-nucleotide polymorphism (SNP)-based genotyping We fine-mapped region generating new down 101 kb eight genes. provided SNP data interval, which could be useful cloning candidate
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ژورنال
عنوان ژورنال: Frontiers in Plant Science
سال: 2021
ISSN: ['1664-462X']
DOI: https://doi.org/10.3389/fpls.2021.691576